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PHERAstar FSX

Powerful and most sensitive HTS plate reader
The PHERAstar® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times.

Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognized by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.

For HTS automation purposes, the PHERAstar FSX offers improved robotic integration capabilities and multi-user control

 

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Features
  • The most sensitive reader in fluorescence intensity and polarization
  • The only plate reader with 5 assay-dedicated detectors
  • Simultaneous Dual Emission, including for Alpha Technology
  • 9 decades luminescence dynamic range
  • Dedicated AlphaScreen®/AlphaLISA®/AlphaPlex™ laser
  • New generation TRF-laser
  • Top and bottom focal height adjustment
  • High-precision injectors with simultaneous reagent injection and detection
  • High-speed absorbance measurements
More details:
Detection Modes: 
  • Fluorescence intensity - incl. FRET
  • Fluorescence polarization: 
  • AlphaScreen®/AlphaLISA®/AlphaPlexTM
  • Luminescence (flash and glow) - incl. BRET
  • Time-resolved fluorescence
  • TR-FRET
  • UV/Vis absorbance spectra

Measurement Modes:
  • Top and bottom reading
  • Endpoint and kinetic
  • Sequential multi-excitation
  • Sequential multi-emission
  • Simultaneous Dual Emission
  • Spectral scanning (absorbance)
  • Real-time ratiometric measurements
  • Well scanning

Microplate Formats:
  • Up to 3456-well plates, user-definable
  • LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier:
  • Robot compatible

Light Sources:
  • High energy xenon flash lamp
  • Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
  • Dedicated laser for TRF and TR-FRET

Detectors:
  • Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes 
  • CCD spectrometer

Wavelength Selection
  • Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application) 
  • For top and bottom reading
  • UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Path Guides
  • Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Z-Adjustment
  • Automatic focal height adjustment (0.1 mm resolution)

Spectral Range
  • Filters
    • 230 - 750 nm or 230 - 900 nm for FI, FP
    • 230 - 750 nm for LUM
    • 230 - 900 nm for TRF
  • Spectrometer
    • 220 - 1000 nm for ABS
Sensitivity
  • FI
  • (top)
    • < 0.15 pM FITC (384sv black, 20 μL)
    • < 0.5 pM FITC (1536 black, 8 μL)
FI
  • (bottom)
    • < 1.0 pM FITC (384g black, 50 μL)
FP
  • < 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
  • < 1.5 mP SD at 1 nM FITC (1536 black, 8 μL) 
TRF
with laser
  • < 5 fM europium (384, 80 μL)
  • < 15 fM europium (1536, 8 μL)  
HTRF®
(black and white microplates)
  • Reader Control Kit (Eu) after 18h (384sv, 20 μL)
  • Delta F > 1100 % (High Calibrator)
  • Delta F > 25 % (Low Calibrator)
LUM
  • < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
  • Dynamic Range: 9 decades 
AlphaScreen®
  • with laser
  • < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS
  • with spectrometer
  • Full spectrum captured in < 1 s / well
  • Selectable spectral resolution: 1, 2, 5, and 10 nm
  • OD range: 0 - 4 OD
  • Accuracy: < 1% at 2 OD
  • Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times
Flying mode
(1 flash) 
  • 14 s (384)
  • 27 s (1536) 
10 flashes
  • 38 s (384)
  • 1 min 52 s (1536) 
50 flashes
  • 1 min 29 s (384)
  • 5 min 18 s (1536) 
Reagent Injection
  • Up to 2 built-in reagent injectors
  • Injection at measurement position (6 to 384-well)
  • Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
  • Variable injection speed up to 420 µL / s
  • Up to four injection events per well
  • Reagent back flushing
Shaking
  • Linear, orbital, and double-orbital with user-definable time and speed
Integrated Barcode Reader
  • Up to three integrated barcode readers
Incubation
  • +5 °C above ambient up to 45 °C
  • The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software
  • Multi-user Reader Control and MARS Data Analysis Software included  - FDA 21 CFR Part 11 compliant
Dimensions
  • Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
LVis Plate
Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy.
Dimensions: conforms to SBS standards for microplates.

Stacker
  • Magazines for up to 50 plates - continuous loading feature
THERMOstar
  • Microplate incubator and shaker

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